AustralieFrV1, Main, Exploration, bibRecord, 009347

Somatostatin decreases voltage-gated Ca2+ currents in GH3 cells through activation of somatostatin receptor 2

Identifieur interne : 009347 ( Main/Exploration ); précédent : 009346; suivant : 009348

Somatostatin decreases voltage-gated Ca2+ currents in GH3 cells through activation of somatostatin receptor 2

Auteurs : Seung-Kwon Yang [Australie] ; Helena C. Parkington [Australie] ; Jacques Epelbaum [France] ; Damien J. Keating [Australie] ; CHEN CHEN [Australie]

Source :

American journal of physiology. Endocrinology and metabolism [ 0193-1849 ] ; 2007.

RBID : Pascal:07-0328815

Descripteurs français

Pascal (Inist)
- Somatostatine, Activation, Récepteur somatostatine, Soustype, Agoniste, Calcium.
Wicri :
- topic : Calcium.

English descriptors

KwdEn :
- Activation, Agonist, Calcium, Somatostatin, Somatostatin receptor, Subtype.

Abstract

The secretion of growth hormone (GH) is inhibited by hypothalamic somatostatin (SRIF) in somatotropes through five subtypes of the somatostatin receptor (SSTR1-SSTR5). We aimed to characterize the subtype(s) of SSTRs involved in the Ca²⁺ current reduction in GH3 somatotrope cells using specific SSTR subtype agonists. We used nystatin-perforated patch clamp to record voltage-gated Ca²⁺ currents, using a holding potential of -80 mV in the presence of K⁺ and Na⁺ channel blockers. We first established the presence of T-, L-, N-, and P/Q-type Ca²⁺ currents in GH3 cells using a variety of channel blockers (Ni⁺, nifedipine, ω-conotoxin GVIA, and ω-agatoxin IVA). SRIF (200 nM) reduced Land N-type but not T- or P/Q-type currents in GH3 cells. A range of concentrations of each specific SSTR agonist was tested on Ca²⁺ currents to find the maximal effective concentration. Activation of SSTR2 with 10^-7 and 10^-8M L-797,976 decreased the voltage-gated Ca²⁺ current and abolished any further decrease by SRIF. SSTR1, SSTR3, SSTR4, and SSTR5 agonists at 10^-7 M did not modify the voltage-gated Ca²⁺ current and did not affect the Ca²⁺ current response to SRIF. These results indicate that SSTR2 is involved mainly in regulating voltage-gated Ca²⁺ currents by SRIF, which contributes to the decrease in intracellular Ca²⁺ concentration and GH secretion by SRIF.

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">The secretion of growth hormone (GH) is inhibited by hypothalamic somatostatin (SRIF) in somatotropes through five subtypes of the somatostatin receptor (SSTR1-SSTR5). We aimed to characterize the subtype(s) of SSTRs involved in the Ca<sup>2+</sup>
 current reduction in GH3 somatotrope cells using specific SSTR subtype agonists. We used nystatin-perforated patch clamp to record voltage-gated Ca<sup>2+</sup>
 currents, using a holding potential of -80 mV in the presence of K<sup>+</sup>
 and Na<sup>+</sup>
 channel blockers. We first established the presence of T-, L-, N-, and P/Q-type Ca<sup>2+</sup>
 currents in GH3 cells using a variety of channel blockers (Ni<sup>+</sup>
, nifedipine, ω-conotoxin GVIA, and ω-agatoxin IVA). SRIF (200 nM) reduced Land N-type but not T- or P/Q-type currents in GH3 cells. A range of concentrations of each specific SSTR agonist was tested on Ca<sup>2+</sup>
 currents to find the maximal effective concentration. Activation of SSTR2 with 10<sup>-7</sup>
 and 10<sup>-8</sup>
M L-797,976 decreased the voltage-gated Ca<sup>2+</sup>
 current and abolished any further decrease by SRIF. SSTR1, SSTR3, SSTR4, and SSTR5 agonists at 10<sup>-7</sup>
 M did not modify the voltage-gated Ca<sup>2+</sup>
 current and did not affect the Ca<sup>2+</sup>
 current response to SRIF. These results indicate that SSTR2 is involved mainly in regulating voltage-gated Ca<sup>2+</sup>
 currents by SRIF, which contributes to the decrease in intracellular Ca<sup>2+</sup>
 concentration and GH secretion by SRIF.</div>
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Somatostatin decreases voltage-gated Ca2+ currents in GH3 cells through activation of somatostatin receptor 2

Somatostatin decreases voltage-gated Ca2+ currents in GH3 cells through activation of somatostatin receptor 2

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri